mouse anti human cd14 fitc  (Bio-Rad)

Journal: Scientific Reports

Article Title: Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellular trap phagocytosis

doi: 10.1038/s41598-024-66916-y

Figure Lengend Snippet: Details of the antibodies used in this study.

Article Snippet: FITC-labeled anti-human CD14 mouse monoclonal antibody , Cat. No. 6603511; Beckman Coulter, Brea, CA, USA.

Techniques:

Journal: Aging (Albany NY)

Article Title: The miR-146b-3p/TNFAIP2 axis regulates cell differentiation in acute myeloid leukaemia

doi: 10.18632/aging.205441

Figure Lengend Snippet: PMA induced THP-1 cell differentiation. ( A ) Flow cytometric determination of the CD11b+ THP-1 cell proportion. ( B ) The CD11b statistical histogram. ( C ) Flow cytometric determination of the CD14+ THP-1 cell proportion. ( D ) The CD14 statistical histogram. ( E ) CD11b and ( F ) CD14 immunofluorescence intensity in THP-1 cells. Normal distribution, ANOVA test, * P < 0.05, **** P < 0.0001.

Article Snippet: Cell differentiation was assessed using immunofluorescence staining with a PE-conjugated mouse anti-human CD11b antibody and an FITC-conjugated mouse anti-human CD14 antibody (BD Bioscience, USA).

Techniques: Cell Differentiation, Immunofluorescence

Journal: Aging (Albany NY)

Article Title: The miR-146b-3p/TNFAIP2 axis regulates cell differentiation in acute myeloid leukaemia

doi: 10.18632/aging.205441

Figure Lengend Snippet: Forced expression of TNFAIP2 significantly induced the differentiation of MOLM-13 cells. ( A ) Flow cytometric determination of the CD11b+ cell proportion in transduced MOLM-13 cells. ( B ) The CD11b statistical histogram. ( C ) Flow cytometric determination of the CD14+ cell proportion in transduced MOLM-13 cells. ( D ) The CD14 statistical histogram. ( E ) The immunofluorescence intensity of CD11b and CD14 in transduced MOLM-13 cells. Normal distribution, t test, ** P < 0.01.

Article Snippet: Cell differentiation was assessed using immunofluorescence staining with a PE-conjugated mouse anti-human CD11b antibody and an FITC-conjugated mouse anti-human CD14 antibody (BD Bioscience, USA).

Techniques: Expressing, Immunofluorescence

Journal: Aging (Albany NY)

Article Title: The miR-146b-3p/TNFAIP2 axis regulates cell differentiation in acute myeloid leukaemia

doi: 10.18632/aging.205441

Figure Lengend Snippet: Knockdown of miR-146b-3p significantly induced the differentiation of MOLM-13 cells. ( A ) Flow cytometric determination of the CD11b+ cell proportion in transduced MOLM-13 cells. ( B ) The CD11b statistical histogram. ( C ) Flow cytometric determination of the CD14+ cell proportion in transduced MOLM-13 cells. ( D ) The CD14 statistical histogram. ( E ) The immunofluorescence intensity of CD11b and CD14 in transduced MOLM-13 cells. Normal distribution, t test, ** P < 0.01.

Article Snippet: Cell differentiation was assessed using immunofluorescence staining with a PE-conjugated mouse anti-human CD11b antibody and an FITC-conjugated mouse anti-human CD14 antibody (BD Bioscience, USA).

Techniques: Immunofluorescence

Journal: Viruses

Article Title: Time-Course Transcriptome Analysis Reveals Distinct Phases and Identifies Two Key Genes during Severe Fever with Thrombocytopenia Syndrome Virus Infection in PMA-Induced THP-1 Cells

doi: 10.3390/v16010059

Figure Lengend Snippet: The results of THP-1 cell sorting using a flow cytometer with CD14-FITC labeling for cell sorting. ( A – C ) represent the gating strategy employed during the sorting of PMA-induced THP-1 cells, labeled as Gate 1 to Gate 3. ( D ) displays a peak map illustrating the cellular composition and distribution resulting from the sorting of PMA-induced THP-1 cells. ( E ) presents the percentage composition of FITC-positive cells obtained from the sorting of PMA-induced THP-1 cells. Similarly, ( F – H ) depict the gating strategy utilized during the sorting of THP-1 cells in the control group. ( I ) exhibits a peak map illustrating the cellular composition and distribution resulting from the sorting of THP-1 cells in the control group. Finally, ( J ) provides the percentage composition of FITC-positive cells obtained from the sorting of THP-1 cells in the control group.

Article Snippet: The antibodies used in this study included Mouse Anti-Human CD14 (FITC marker) (BD) (Franklin Lakes, NJ, USA).

Techniques: FACS, Flow Cytometry, Labeling